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A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe

journal contribution
posted on 2023-06-07, 13:56 authored by J. A. Farah, E. Hartsuiker, Ken'ichi Mizuno, K. Ohta, G. R. Smith
Palindromic sequences can form hairpin and cruciform structures that pose a threat to genome integrity. We found that a 160-bp palindrome (an inverted repeat of 80 bp) conferred a mitotic recombination hotspot relative to a control nonpalindromic sequence when inserted into the ade6 gene of Schizosaccharomyces pombe. The hotspot activity of the palindrome, but not the basal level of recombination, was abolished by a rad50 deletion, by a rad50S "separation of function" mutation, or by a rad32-D25A mutation in the nuclease domain of the Rad32 protein, an Mre11 homolog. We propose that upon extrusion of the palindrome the Rad50.Rad32 nuclease complex recognizes and cleaves the secondary structure thus formed and generates a recombinogenic break in the DNA.

History

Publication status

  • Published

Journal

Genetics

ISSN

0016-6731

Issue

1

Volume

161

Page range

461-468

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2007-05-03

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