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The COP9/signalosome complex is conserved in fission yeast and has a role in S phase
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posted on 2023-06-08, 00:37 authored by Kirsten E Mundt, Joanne Porte, Jo Murray, Constantinos Brikos, Per U Christensen, Thomas Caspari, Iain M Hagan, Jonathan B A Millar, Viesturs Simanis, Kay Hofmann, Antony CarrAntony CarrThe COP9/signalosome complex is conserved from plant to mammalian cells. In Arabidopsis, it regulates the nuclear abundance of COP1, a transcriptional repressor of photomorphogenic development [1] [2]. All COP (constitutive photomorphogenesis) mutants inappropriately express genes that are normally repressed in the dark. Eight subunits (Sgn1-Sgn8) of the homologous mammalian complex have been purified [3] [4]. Several of these have been previously identified through genetic or protein interaction screens. No coherent model for COP9/signalosome function has yet emerged, but a relationship with cell-cycle progression by transcriptional regulation, protein localisation or protein stability is possible. Interestingly, the COP9/signalosome subunits possess domain homology to subunits of the proteasome regulatory lid complex [5] [6]. Database searches indicate that only Sgn5/JAB1 is present in Saccharomyces cerevisiae, precluding genetic analysis of the complex in cell-cycle regulation. Here we identify a subunit of the signalosome in the fission yeast Schizosaccharomyces pombe through an analysis of the DNA-integrity checkpoint. We provide evidence for the conservation of the COP9/signalosome complex in fission yeast and demonstrate that it functions during S-phase progression.
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Publication status
- Published
Journal
Current BiologyPublisher
Current BiologyExternal DOI
Issue
23Volume
9Page range
1427-1430ISBN
0960-9822Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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- No
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- Yes
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2012-02-06Usage metrics
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