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Identification and characterisation of Schizosaccharomyces pombe cyclophilin 3 and its human homologue cyclophilin 20

journal contribution
posted on 2023-06-08, 00:52 authored by Trevor J Pemberton, Stuart L Rulten, John E Kay
We have identified nine cyclophilins encoded in the genome of the fission yeast Schizosaccharomyces pombe (Sp). Cyclophilin 3 is an orthologue of hUSA-CyP, which is associated with Prp4/Prp3 in the [U4/U6·U5] snRNP complex and Prp18, both of which are components of the pre-mRNA splicing machinery. PPIase assays have shown SpCyp3 and hUSA-CyP to have comparable activity and substrate specificity, but SpCyp3 has a reduced sensitivity to CsA correlating with a difference in the catalytic site. Prp3, Prp4 and Prp18 proteins exist in S. pombe and nuclear localisation of SpCyp3 has been shown, indicating conservation of function between hUSA-CyP and SpCyp3.

History

Publication status

  • Published

Journal

Journal of Chromatography B

ISSN

1570-0232

Publisher

Elsevier

Issue

1-2

Volume

786

Page range

81-91

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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