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End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae
During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.
History
Publication status
- Published
Journal
Methods in Molecular BiologyISSN
10643745Publisher
Humana Press Inc.External DOI
Volume
557Page range
183-195Book title
Methods in Molecular BiologyDepartment affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
Source Type: Book series (ISBN: 978-193411566-4), Document Type: ArticleFull text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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