journal.ppat.1004038.pdf (2.77 MB)
Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria
journal contribution
posted on 2023-06-08, 17:55 authored by Delmiro Fernandez-Reyes, Peter Nilsson, Julie Bachmann, Florence Burte, Ianina Conte, Biobele J Brown, Adebola E Orimadegun, Wasiu A. Ajetunmobi, Nathaniel K Afolabi, Francis Akinkunmi, Samuel Omokhodion, Felix O Akinbami, Wuraola A Shokunbi, Caroline Kampf, Yudi Pawitan, Mathias Uhlén, Olugbemiro Sodeinde, Jochen M Schwenk, Mats WahlgrenSystemic inflammation and sequestration of parasitized erythrocytes are central processes in the pathophysiology of severe Plasmodium falciparum childhood malaria. However, it is still not understood why some children are more at risks to develop malaria complications than others. To identify human proteins in plasma related to childhood malaria syndromes, multiplex antibody suspension bead arrays were employed. Out of the 1,015 proteins analyzed in plasma from more than 700 children, 41 differed between malaria infected children and community controls, whereas 13 discriminated uncomplicated malaria from severe malaria syndromes. Markers of oxidative stress were found related to severe malaria anemia while markers of endothelial activation, platelet adhesion and muscular damage were identified in relation to children with cerebral malaria. These findings suggest the presence of generalized vascular inflammation, vascular wall modulations, activation of endothelium and unbalanced glucose metabolism in severe malaria. The increased levels of specific muscle proteins in plasma implicate potential muscle damage and microvasculature lesions during the course of cerebral malaria.
Funding
U117585869; MRC
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Publication status
- Published
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- Published version
Journal
PLoS PathogensISSN
1553-7366Publisher
Public Library of ScienceExternal DOI
Issue
4Volume
10Article number
e1004038Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2014-07-24First Open Access (FOA) Date
2014-07-24First Compliant Deposit (FCD) Date
2014-07-24Usage metrics
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