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Tel1ATM dictates the replication timing of short yeast telomeres

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posted on 2023-06-08, 20:25 authored by Carol Cooley, Anoushka Dave, Mansi Garg, Alessandro BianchiAlessandro Bianchi
Telomerase action is temporally linked to DNA replication. Although yeast telomeres are normally late replicating, telomere shortening leads to early firing of subtelomeric DNA replication origins. We show that double-strand breaks flanked by short telomeric arrays cause origin firing early in S phase at late-replicating loci and that this effect on origin firing time is dependent on the Tel1ATM checkpoint kinase. The effect of Tel1ATM on telomere replication timing extends to endogenous telomeres and is stronger than that elicited by Rif1 loss. These results establish that Tel1ATM specifies not only the extent but also the timing of telomerase recruitment.

Funding

Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi); G0129; MRC-MEDICAL RESEARCH COUNCIL; G0701428

Mechanism of telomerase activation and inhibition at fission yeast telomeres; G0247; CANCER RESEARCH UK; C28567/A12720

History

Publication status

  • Published

File Version

  • Published version

Journal

EMBO Reports

ISSN

1469-221X

Publisher

Embo Press

Issue

10

Volume

15

Page range

1093-1101

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2015-03-25

First Open Access (FOA) Date

2015-03-30

First Compliant Deposit (FCD) Date

2015-03-30

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