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Time to overcome fluconazole resistant Candida isolates: solid lipid nanoparticles as a novel antifungal drug delivery system

journal contribution
posted on 2023-06-09, 06:12 authored by Maryam Moazeni, Hamid Reza Kelidari, Majid Saeedi, Ketayoun Morteza-Semnani, Mojtaba Nabili, Atefeh Abdollahi Gohar, Jafar Akbari, Ensieh Lotfali, Ali Nokhodchi
Antifungal therapy results in complications in management due to changes in the patterns of epidemiology and drug susceptibility of invasive fungal infections. In this study, we prepared fluconazole-loaded solid lipid nanoparticles (FLZ-SLNs) and investigated the efficacy of the optimal formulation on fluconazole (FLZ)-resistant strains of several Candida species. FLZ-SLN was produced using probe ultrasonication techniques. The morphology of the obtained SLNs was characterized by field emission scanning electron microscopy. The minimum inhibitory concentrations for the new formulations against fluconazole-resistant strains of Candida were investigated using CLSI document M27-A3. The FLZ-SLNs presented a spherical shape with a mean diameter, zeta potential and entrapment efficiency of 84.8 nm, -25 mV and 89.6%, respectively. The drug release from FLZ-SLNs exhibited burst release behaviour at the initial stage (the first 30 min) followed by a sustained release over 24 h FLZ-resistant yeast strains behaved as susceptible strains after treatment with FLZ-SLNs (=8 µg/ml). The MIC50 drug concentrations were 2 µg/ml, 1 µg/ml and 2 µg/ml for FLZ-resistant strains of Candida albicans, Candida parapsilosis and Candida glabrata, respectively. In this study, we evaluated novel delivery systems for combating Candida strains that exhibit low susceptibility against the conventional formulation of FLZ as a first-line treatment

History

Publication status

  • Published

File Version

  • Published version

Journal

Colloids and Surfaces B: Biointerfaces

ISSN

0927-7765

Publisher

Elsevier

Volume

142

Page range

400-407

Department affiliated with

  • Chemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2017-05-08

First Compliant Deposit (FCD) Date

2021-03-09

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