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[Protocol]: Transformation of schizosaccharomyces pombe: protoplast procedure

journal contribution
posted on 2023-06-09, 09:13 authored by Jo Murray, Adam WatsonAdam Watson, Antony CarrAntony Carr
Transformation of Schizosaccharomyces pombe with DNA requires the conditioning of cells to promote DNA uptake followed by cell growth under conditions that select and maintain the plasmid or integration event. The three main methodologies are electroporation, treatment with lithium cations, and transformation of protoplasts. The protocol for protoplast transformation, which is described here, is more complicated than those for electroporation or lithium acetate and thus less often used. However, for some strains, it remains the only reliable protocol.

Funding

Smc5/6 and replication fork stability; G0179; MRC-MEDICAL RESEARCH COUNCIL; G0901011

Replication stalling in a palindrome; G0181; ASSOCIATION FOR INTERNATIONAL CANCER RESEARCH; 10-0273

History

Publication status

  • Published

File Version

  • Accepted version

Journal

Cold Spring Harbour Protocols

ISSN

1559-6095

Publisher

Cold Spring Harbor Laboratory Press

Issue

4

Volume

2016

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Research groups affiliated with

  • Genome Damage and Stability Centre Publications

Full text available

  • No

Peer reviewed?

  • No

Legacy Posted Date

2017-12-12

First Compliant Deposit (FCD) Date

2017-12-12

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