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Two-photon fluorescence imaging with 30 fs laser system tunable around 1 micron

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posted on 2023-06-09, 09:27 authored by Bojan Resan, Rodrigo Amador Aviles-EspinosaRodrigo Amador Aviles-Espinosa, Sarah Kurmulis, Jacob Licea-Rodriguez, Felix Brunner, Andreas Rohrbacher, David Artigas, Pablo Loza-Alvarez, Kurt J Weingarten
We developed a low-cost, low-noise, tunable, high-peak-power, ultrafast laser system based on a SESAM-modelocked, solid-state Yb tungstate laser plus spectral broadening via a microstructured fiber followed by pulse compression. The spectral selection, tuning, and pulse compression are performed with a simple prism compressor. The output pulses are tunable from 800 to 1250 nm, with the pulse duration down to 25 fs, and average output power up to 150 mW, at 80 MHz pulse repetition rate. We introduce the figure of merit (FOM) for the two-photon and multi-photon imaging (or other nonlinear processes), which is a useful guideline in discussions and for designing the lasers for an improved microscopy signal. Using a 40 MHz pulse repetition rate laser system, with twice lower FOM, we obtained high signal-to-noise ratio two-photon fluorescence images with or without averaging, of mouse intestine section and zebra fish embryo. The obtained images demonstrate that the developed system is capable of nonlinear (TPE, SHG) imaging in a multimodal operation. The system could be potentially used in a variety of other techniques including, THG, CARS and applications such as nanosurgery.

History

Publication status

  • Published

File Version

  • Published version

Journal

Optics Express

ISSN

1094-4087

Publisher

Optical Society of America

Issue

13

Volume

22

Page range

16456-16461

Department affiliated with

  • Engineering and Design Publications

Research groups affiliated with

  • Sensor Technology Research Centre Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2017-12-19

First Open Access (FOA) Date

2017-12-19

First Compliant Deposit (FCD) Date

2017-12-19

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