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The telomere-binding protein Taz1p as a target for modification by a SUMO-1 homologue in fission yeast

journal contribution
posted on 2023-06-07, 13:52 authored by K Spink, J C Ho, K Tanaka, Felicity Watts, Anna Chambers
In fission yeast (Schizosaccharomyces pombe) the homologue of the mammalian SUMO-1 ubiquitin-like modifier is encoded by the pmt3 gene. A two-hybrid screen using the telomere-binding protein Taz1p as bait identified Pmt3p as an interacting factor. In vitro experiments using purified components of the fission yeast Pmt3p modification system demonstrated that Taz1p could be modified directly by Pmt3p. The amino acid sequence of Taz1p contains a close match to the consensus modification site for SUMO-1, and a PEST sequence similar to those found in established SUMO-1 targets. Although previous experiments have identified an increase in telomere length as one consequence of the pmt3--genotype, we could not detect Pmt3p modification of Taz1p in protein extracts made from exponentially growing haploid cells or any effect of Pmt3p on the localization of GFP-Taz1p at discrete foci in the haploid cell nucleus.

History

Publication status

  • Published

Journal

Biochemical Genetics

ISSN

0006-2928

Publisher

Springer Verlag

Issue

3-4

Volume

43

Page range

103-17

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

GDSC174

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2007-03-19