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Domain structure, localization, and function of DNA polymerase ?, defective in xeroderma pigmentosum variant cells

journal contribution
posted on 2023-06-07, 20:16 authored by Patricia Kannouche, Bernard C Broughton, Marcel Volker, Fumio Hanaoka, Leon H F Mullenders, Alan LehmannAlan Lehmann
DNA polymerase ¿ carries out translesion synthesis past UV photoproducts and is deficient in xeroderma pigmentosum (XP) variants. We report that pol¿ is mostly localized uniformly in the nucleus but is associated with replication foci during S phase. Following treatment of cells with UV irradiation or carcinogens, it accumulates at replication foci stalled at DNA damage. The C-terminal third of pol¿ is not required for polymerase activity. However, the C-terminal 70 aa are needed for nuclear localization and a further 50 aa for relocalization into foci. Pol¿ truncations lacking these domains fail to correct the defects in XP-variant cells. Furthermore, we have identified mutations in two XP variant patients that leave the polymerase motifs intact but cause loss of the localization domains.

History

Publication status

  • Published

Journal

Genes & Development

ISSN

0890-9369

Publisher

Cold Spring Harbor Laboratory Press

Issue

2

Volume

15

Page range

158-172

Pages

15.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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