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Postreplication Repair and PCNA Modification in Schizosaccharomyces pombe

journal contribution
posted on 2023-06-07, 20:51 authored by Jonathan Frampton, Anja Irmisch, Catherine M Green, Andrea Neiss, Michelle Trickey, Helle D Ulrich, Kanji Furuya, Felicity Watts, Antony CarrAntony Carr, Alan LehmannAlan Lehmann
Ubiquitination of proliferating cell nuclear antigen (PCNA) plays a crucial role in regulating replication past DNA damage in eukaryotes, but the detailed mechanisms appear to vary in different organisms. We have examined the modification of PCNA in Schizosaccharomyces pombe. We find that, in response to UV irradiation, PCNA is mono- and poly-ubiquitinated in a manner similar to that in Saccharomyces cerevisiae. However in undamaged Schizosaccharomyces pombe cells, PCNA is ubiquitinated in S phase, whereas in S. cerevisiae it is sumoylated. Furthermore we find that, unlike in S. cerevisiae, mutants defective in ubiquitination of PCNA are also sensitive to ionizing radiation, and PCNA is ubiquitinated after exposure of cells to ionizing radiation, in a manner similar to the response to UV-irradiation. We show that PCNA modification and cell cycle checkpoints represent two independent signals in response to DNA damage. Finally, we unexpectedly find that PCNA is ubiquitinated in response to DNA damage when cells are arrested in G2

History

Publication status

  • Published

Journal

Molecular Biology of the Cell

ISSN

1059-1524

Publisher

American Society for Cell Biology

Issue

7

Volume

17

Page range

2976-2985

Pages

8.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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