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Effective and reproducible protocols for in vitro culturing and plant regeneration of the physiological model plant Ramonda myconi (L.) Rchb

journal contribution
posted on 2023-06-07, 20:51 authored by Sándor Tóth, Peter Scott, Seppo Sorvari, Ottó Toldi
The first procedures have been established for in vitro micropropagation and plant regeneration of the desiccation-tolerant plant Ramonda myconi (L.) Rchb. An integrated optimisation of salt, antioxidant and growth regulator composition, and potassium-phosphate buffer-mediated pH stabilisation of culture media resulted in an effective tissue culture system that prevented the undesirable hyperhydricity and tissue necrosis typical of Ramonda tissue cultures. We examined whether there is any similarity in tissue culturing between the two resurrection plants Craterostigma plantagineum and R. myconi as a consequence of their common physiological specialisation. The fact that regenerated plants developed on dedifferentiated cell cultures, which is a precondition of Agrobacterium-mediated gene transfer, could mean an applicable basis for the optimisation of the genetic transformation of Ramonda species.

History

Publication status

  • Published

Journal

Plant Science

ISSN

0168-9452

Issue

4

Volume

166

Page range

1027-1034

Pages

8.0

Department affiliated with

  • Biology and Environmental Science Publications

Notes

This work was performed in my laboratory by a Postdoc work. Some final experiments were completed by the postdoc in Hungary hence the collaborators.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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