Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex

Taylor, Elaine M, Copsey, Alice C, Hudson, Jessica J, Vidot, Susanne and Lehmann, Alan R (2008) Identification of the proteins, including MAGEG1, that make up the human SMC5-6 protein complex. Molecular and Cellular Biology, 28 (4). pp. 1197-1206. ISSN 0270-7306

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The SMC protein complexes play important roles in chromosome dynamics. The function of the SMC5-6 complex remains unclear, though it is involved in resolution of different DNA structures by recombination. We have now identified and characterized the four non-SMC components of the human complex and in particular demonstrated that the MAGEG1 protein is part of this complex. MAGE proteins play important but as yet undefined roles in carcinogenesis, apoptosis, and brain development. We show that, with the exception of the SUMO ligase hMMS21/hNSE2, depletion of any of the components results in degradation of all the other components. Depletion also confers sensitivity to methyl methanesulfonate. Several of the components are modified by sumoylation and ubiquitination.

Item Type: Article
Keywords: Amino Acid Sequence Carrier Proteins/chemistry/metabolism Cell Cycle Proteins/chemistry/*metabolism Cell Survival/drug effects DNA Damage Hela Cells Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins/*metabolism Ligases/chemistry/*metabolism Mass Spectrometry Methyl Methanesulfonate/pharmacology Molecular Sequence Data Protein Processing, Post-Translational/drug effects Schizosaccharomyces pombe Proteins/chemistry/metabolism Small Ubiquitin-Related Modifier Proteins/metabolism Thermodynamics Ubiquitination/drug effects
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > Q Science (General)
Depositing User: Gee Wheatley
Date Deposited: 17 Jul 2008
Last Modified: 11 May 2018 15:04

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