In vitro study of the effects of cadmium on the activation of the estrogen response element using the YES screen

Denier, Xavier, Couteau, Jérome, Baudrimont, Magalie, Hill, Elizabeth M, Rotchell, Jeanette and Minier, Christophe (2008) In vitro study of the effects of cadmium on the activation of the estrogen response element using the YES screen. Marine Environmental Research, 66 (1). pp. 108-110. ISSN 0141-1136

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Abstract

Estrogenic potential of environmental samples is frequently assessed using receptor-based functional assays. Using the yeast estrogen screen (YES) developed by Routledge and Sumpter, we assessed the ability of cadmium to activate the estrogen receptor-mediated response. No induced transcriptional activity was observed with a range of CdCl2 concentrations (1 nm¿1 mM). But, when combining cadmium with the model compound 17ß-estradiol, cadmium was able to significantly potentiate the induced estrogenic response for concentrations ranging from 15 nM to 1 µM. A maximal effect was observed at 0.5 µM with a ten fold reduction of the 17ß-estradiol EC50.

Item Type: Article
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Depositing User: Elizabeth Hill
Date Deposited: 06 Feb 2012 18:44
Last Modified: 18 Jun 2012 13:11
URI: http://srodev.sussex.ac.uk/id/eprint/18100
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