Control of single-molecule fluorescence dynamics by stimulated emission depletion

Osborne, Mark A, Marsh, Richard J and Bain, Angus J (2003) Control of single-molecule fluorescence dynamics by stimulated emission depletion. In: Conference on Nanocrystals and Organic and Hybrid Nanomaterials. SPIE-international Proceeding of the Society of Optical Engineering, SAN DIEGO, CA.

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The feasibility of manipulating the single molecule absorption-emission cycle using picosecond stimulated emission depletion (STED) is investigated using a stochastic computer simulation. In the simulation the molecule is subjected to repeated excitation and depletion events using time delayed pairs of excitation (PUMP) and depletion (DUMP) pulses derived from a high repetition rate pulsed laser system. The model is used to demonstrate that a significant and even substantial reduction in the occurrence of 'dark states' in the fluorescence emission can be achieved using stimulated emission depletion. Variation in the PUMP-DUMP window allows precise control of the fluorescence yield with substantial increases in the fluorescence intensity observed at early PUMP-DUMP delays.

Item Type: Conference or Workshop Item (Paper)
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: Mark Osborne
Date Deposited: 06 Feb 2012 19:03
Last Modified: 21 Mar 2012 09:40
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