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Phase-dependent molecular requirements for memory reconsolidation: differential roles for protein synthesis and protein kinase A activity

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posted on 2023-06-07, 22:26 authored by George KemenesGeorge Kemenes, Ildiko KemenesIldiko Kemenes, Maximilian Michel, Andrea Papp, Uli Mueller
After consolidation, a process that requires gene expression and protein synthesis, memories are stable and highly resistant to disruption by amnestic influences. Recently, consolidated memory has been shown to become labile again after retrieval and to require a phase of reconsolidation to be preserved. New findings, showing that the dependence of reconsolidation on protein synthesis decreases with the age of memory, point to changing molecular requirements for reconsolidation during memory maturation. We examined this possibility by comparing the roles of protein synthesis (a general molecular requirement for memory consolidation) and the activation of protein kinase A (PKA) (a specific molecular requirement for memory consolidation), in memory reconsolidation at two time points after training. Using associative learning in Lymnaea, we show that reconsolidation after the retrieval of consolidated memory at both 6 and 24 h requires protein synthesis. In contrast, only reconsolidation at 6 h after training, but not at 24 h, requires PKA activity, which is in agreement with the measured retrieval-induced PKA activation at 6 h. This phase-dependent differential molecular requirement for reconsolidation supports the notion that even seemingly consolidated memories undergo further selective molecular maturation processes, which may only be detected by analyzing the role of specific pathways in memory reconsolidation after retrieval.

History

Publication status

  • Published

File Version

  • Published version

Journal

Journal of Neuroscience

ISSN

0270-6474

Publisher

Society for Neuroscience

Issue

23

Volume

26

Page range

6298-6302

Pages

5.0

Department affiliated with

  • Neuroscience Publications

Notes

Principal investigator and corresponding author. Designed and directed the research, performed many of the experiments himself and took a major part in the writing of the paper. MM was a postgraduate student, AP was an RA in his laboratory, IK was an internal collaborator, UM was an international collaborator.

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

First Open Access (FOA) Date

2016-03-22

First Compliant Deposit (FCD) Date

2016-08-17

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