Morphological observation of nuclear sub-localization of human angiogenin in HUVECs

Wu, Jie, Zhang, Hong-Bin, Wang, Jie, Yang, Tai-Cheng, Xian, Jiang, Yang, Chuan-Hong and Lai, Huang-Wen (2008) Morphological observation of nuclear sub-localization of human angiogenin in HUVECs. Biotechnology Letters, 30 (6). pp. 989-993. ISSN 0141-5492

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Abstract

Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hybridoma cells stably and specifically binds to angiogenin. The fused protein angiogenin-EGF was then expressed in HUVECs, and the subcellular localization of the recombinant protein was determined by confocal microscopy and TEM assay. Recombinant angiogenin was found to mainly concentrate in the pars granulosa of the nucleus, where the protein accumulates to form ribonucleoprotein particles. © 2008 Springer Science+Business Media B.V.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Depositing User: Tai Yang
Date Deposited: 06 Feb 2012 19:20
Last Modified: 30 Mar 2012 08:22
URI: http://srodev.sussex.ac.uk/id/eprint/20133
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