Localization of Y‐Family Polymerases and the DNA Polymerase Switch in Mammalian Cells

Kannouche, Patricia and Lehmann, Alan (2006) Localization of Y‐Family Polymerases and the DNA Polymerase Switch in Mammalian Cells. Methods in Enzymology, 408. pp. 407-415. ISSN 0076-6879

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Abstract

During translesion synthesis past sites of damaged DNA, specialized Y-family polymerases are employed by the cell to replace the high stringency replicative polymerases and synthesize DNA past the damaged site. These polymerases are localized in replication factories during the S phase of the cell cycle. When progress of the replication fork is blocked, the polymerase accessory protein, proliferating cell nuclear antigen (PCNA), becomes ubiquitinated and the monoubiquitinated PCNA has an increased affinity for Y-family DNA polymerase ¿ (pol¿). This chapter describes methods for visualizing the polymerases in replication factories, for analyzing the ubiquitination status of PCNA, and for measuring its interaction with pol¿ in chromatin extracts.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Alan Lehmann
Date Deposited: 06 Feb 2012 19:27
Last Modified: 30 Mar 2012 09:11
URI: http://srodev.sussex.ac.uk/id/eprint/20673
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