Kinetics of re-establishing H3K79 methylation marks in global human chromatin.

Sweet, Steve, Li, M, Thomas, PM, Durbin, K.R. and Kelleher, N.L (2010) Kinetics of re-establishing H3K79 methylation marks in global human chromatin. Journal of Biological Chemistry, 285 (43). pp. 32778-32786. ISSN 00219258

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Abstract

We employ a stable isotope strategy wherein both histones and their methylations are labeled in synchronized human cells. This allows us to differentiate between old and new methylations on pre-existing versus newly synthesized histones. The strategy is implemented on K79 methylation in an isoform-specific manner for histones H3.1, H3.2, and H3.3. Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the rate of establishing the K79 methylation is the same for all three isoforms. Surprisingly, we find that pre-existing "old" histones continue to be K79-monomethylated and -dimethylated at a rate equal to the newly synthesized histones. These observations imply that some degree of positional "scrambling" of K79 methylation occurs through the cell cycle.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Related URLs:
Depositing User: Steve Sweet
Date Deposited: 06 Feb 2012 19:33
Last Modified: 11 Oct 2012 09:38
URI: http://srodev.sussex.ac.uk/id/eprint/21233
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