Occurrence of rare somatomammotrophs in ovine anterior pituitary tissue studied by immunogold labelling and electron microscopy

Thorpe, J R, Ray, K P and Wallis, M (1990) Occurrence of rare somatomammotrophs in ovine anterior pituitary tissue studied by immunogold labelling and electron microscopy. Journal of Endocrinology, 124. pp. 67-73.

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Abstract

Prolactin and GH are distinct hormones that have been conventionally thought to be produced and secreted by separate cells in the anterior pituitary gland. Recently it has been suggested that some cells (somatomammotrophs) may secrete both hormones. We have examined the occurrence of somatomammotrophs in sheep anterior pituitary tissue using immunogold labelling. Of a number of procedures used, double labelling using first antibodies raised in different species proved the least susceptible to apparent co-localization of hormones due to artifacts. Using this approach it was shown that a large proportion of the cells in the sheep anterior pituitary glands examined were mammotrophs or somatotrophs, showing no significant co-localization of GH and prolactin. Of 1800 cells examined, only two were somatomammotrophs. One of these, from a female animal, contained GH and prolactin in different granules within the same cell. The other, from a male animal, showed co-localization of these two hormones within the same granules.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Julian Thorpe
Date Deposited: 06 Feb 2012 19:43
Last Modified: 30 Nov 2012 17:04
URI: http://srodev.sussex.ac.uk/id/eprint/21894
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