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A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway
journal contribution
posted on 2023-06-08, 00:47 authored by Maria A Marchetti, Sanjay Kumar, Edgar Hartsuiker, Mohamed Maftahi, Antony CarrAntony Carr, Greg A Freyer, William C Burhans, Joel A HubermanThe eukaryotic intra-S-phase checkpoint, which slows DNA synthesis in response to DNA damage, is poorly understood. Is DNA damage recognized directly, or indirectly through its effects on replication forks? Is the slowing of S phase in part because of competition between DNA synthesis and recombination/repair processes? The results of our genetic analyses of the intra-S-phase checkpoint in the fission yeast, Schizosaccharomyces pombe, suggest that the slowing of S phase depends weakly on the helicases Rqh1 and Srs2 but not on other recombination/repair pathways. The slowing of S phase depends strongly on the six checkpoint-Rad proteins, on Cds1, and on Rad4/Cut5 (similar to budding yeast Dpb11, which interacts with DNA polymerase ¿) but not on Rhp9 (similar to budding yeast Rad9, necessary for direct damage recognition). These results suggest that, in fission yeast, the signal activating the intra-S-phase checkpoint is generated only when replication forks encounter DNA damage.
History
Publication status
- Published
Journal
Proceedings of the National Academy of SciencesISSN
1091-6490Publisher
National Academy of SciencesExternal DOI
Issue
11Volume
99Page range
7472-7477Pages
6.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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