Translation initiation factors and active sites of protein synthesis co-localise at the leading edge of migrating MRC5 fibroblasts

Morley, Simon, Brocard, Michele, Davide, Alexandre and Willett, Mark (2011) Translation initiation factors and active sites of protein synthesis co-localise at the leading edge of migrating MRC5 fibroblasts. Biochemical Journal, 438 (1). pp. 217-227. ISSN 0264-6021

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Abstract

Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science
Depositing User: Simon Morley
Date Deposited: 06 Feb 2012 20:07
Last Modified: 05 Oct 2012 09:18
URI: http://srodev.sussex.ac.uk/id/eprint/24170
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