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Ipl1/Aurora B kinase coordinates synaptonemal complex disassembly with cell cycle progression and crossover formation in budding yeast meiosis

journal contribution
posted on 2023-06-07, 15:30 authored by Philip Jordan, Alice CopseyAlice Copsey, Louise Newnham, Elizabeth Kolar, Michael Lichten, Eva Hoffmann
Several protein kinases collaborate to orchestrate and integrate cellular and chromosomal events at the G2/M transition in both mitotic and meiotic cells. During the G2/M transition in meiosis, this includes the completion of crossover recombination, spindle formation, and synaptonemal complex (SC) breakdown. We identified Ipl1/Aurora B kinase as the main regulator of SC disassembly. Mutants lacking Ipl1 or its kinase activity assemble SCs with normal timing, but fail to dissociate the central element component Zip1, as well as its binding partner, Smt3/SUMO, from chromosomes in a timely fashion. Moreover, lack of Ipl1 activity causes delayed SC disassembly in a cdc5 as well as a CDC5-inducible ndt80 mutant. Crossover levels in the ipl1 mutant are similar to those observed in wild type, indicating that full SC disassembly is not a prerequisite for joint molecule resolution and subsequent crossover formation. Moreover, expression of meiosis I and meiosis II-specific B-type cyclins occur normally in ipl1 mutants, despite delayed formation of anaphase I spindles. These observations suggest that Ipl1 coordinates changes to meiotic chromosome structure with resolution of crossovers and cell cycle progression at the end of meiotic prophase.

History

Publication status

  • Published

File Version

  • Published version

Journal

Genes & Development

ISSN

0890-9369

Publisher

Cold Spring Harbor Laboratory Press

Issue

18

Volume

23

Page range

2237-2251

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

GDSC297

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2010-07-19

First Open Access (FOA) Date

2023-02-22

First Compliant Deposit (FCD) Date

2023-02-22

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