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Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

journal contribution
posted on 2023-06-08, 05:28 authored by David D U Li, David Tyndall, Richard Walker, Justin Richardson, Robert K Henderson, Jochen Arlt, David Stoppa, Edoardo Charbon
A high-speed and hardware-only algorithm using a center of mass method (CMM) has been proposed for single-detector fluorescence lifetime sensing applications [J. Biomedical Opt. 15, 2010]. This algorithm is now implemented on a field programmable gate array (FPGA) to provide fast lifetime estimates from a 3232 low dark count 0.13m CMOS single-photon avalanche diode (SPAD) plus time-to-digital converter (TDC) array. A simple look-up table (LUT) is included to enhance the lifetime resolvability range and photon economics, making it comparable to the commonly used least-square method (LSM) and maximum-likelihood estimation (MLE) based software. To demonstrate its performance, a widefield microscope was adapted to accommodate the SPAD array and image different test samples. Fluorescence lifetime imaging microscopy (FLIM) on fluorescent beads in Rhodamine 6G at a frame rate of 50fps is also shown.

History

Publication status

  • Published

Journal

Journal of Biomedical Optics

ISSN

1083-3668

Publisher

Society of Photo-optical Instrumentation Engineers

Issue

9

Volume

16

Page range

096012

Department affiliated with

  • Engineering and Design Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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