Shin, Jin, Choi, Eun Shik, Kim, Hyun Soo, Ho, J C, Watts, Felicity Z, Park, Sang D and Jang, Yeun Kyu (2005) SUMO modification is involved in the maintenance of heterochromatin stability in fission yeast. Molecular Cell, 19 (6). pp. 817-828. ISSN 1097-2765

Full text not available from this repository.

Abstract

Several studies have suggested that SUMO may participate in the regulation of heterochromatin, but direct evidence is lacking. Here, we present a direct link between sumoylation and heterochromatin stability. SUMO deletion impaired silencing at heterochromatic regions and induced histone H3 Lys4 methylation, a hallmark of active chromatin in fission yeast. Our findings showed that the SUMO-conjugating enzyme Hus5/Ubc9 interacted with the conserved heterochromatin proteins Swi6, Chp2 (a paralog of Swi6), and Clr4 (H3 Lys9 methyltransferase). Moreover, chromatin immunoprecipitation (ChIP) revealed that Hus5 was highly enriched in heterochromatic regions in a heterochromatin-dependent manner, suggesting a direct role of Hus5 in heterochromatin formation. We also found that Swi6, Chp2, and Clr4 themselves can be sumoylated in vivo and defective sumoylation of Swi6 or Chp2 compromised silencing. These results indicate that Hus5 associates with heterochromatin through interactions with heterochromatin proteins and modifies substrates whose sumoylations are required for heterochromatin stability, including heterochromatin proteins themselves.

Item Type:	Article
Schools and Departments:	School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User:	Felicity Watts
Date Deposited:	06 Feb 2012 20:18
Last Modified:	25 Jun 2015 10:41
URI:	http://srodev.sussex.ac.uk/id/eprint/25322

📧 Request an update