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Cloning of the Retinoblastoma cDNA from the Japanese medaka (Oryzias latipes) and preliminary evidence of mutational alterations in chemically-induced retinoblastomas

journal contribution
posted on 2023-06-08, 06:05 authored by J M Rotchell, J B Blair, J-K Shim, W E Hawkins, G K Ostrander
We have cloned a medaka homolog of the human retinoblastoma (Rb) susceptibility gene. The medaka Rb cDNA encodes a predicted protein of 909 amino acids. DNA sequence analysis with other vertebrate Rb sequences demonstrates that the medaka Rb cDNA is highly conserved in regions of functional importance. An antibody raised against an epitope of the human pRb recognizes the protein product of the medaka Rb gene, detecting a 105 kDa protein in all tissues examined and at differential levels for the stages of embryonic development studied. The sequence reported herein, combined with the high degree of conservation observed in critical domains, has also facilitated a preliminary investigation of the molecular etiology of chemically-induced retinoblastoma. The mutational alterations characterized suggest that medaka may provide a novel model and, thus, provide additional insight into the human retinoblastoma condition.

History

Publication status

  • Published

Journal

Gene

ISSN

0378-1119

Issue

1-2

Volume

263

Page range

231-237

Pages

7.0

Department affiliated with

  • Biology and Environmental Science Publications

Notes

This is a novel interdisciplinary result, describing the development of a fish model for human retinoblastoma. I was funded by NIH (as a postdoc at Johns Hopkins University) and BBSRC (as principal investigator), and is recognition that fish can be a suitable surrogate for studying certain cancers.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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