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The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis
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posted on 2023-06-08, 06:19 authored by Thomas A Hawkins, Anna-Pavlina Haramis, Christelle Etard, Chrisostomos ProdromouChrisostomos Prodromou, Cara K Vaughan, Rachel Ashworth, Saikat Ray, Martine Behra, Nigel Holder, William S Talbot, Laurence PearlLaurence Pearl, Uwe Strähle, Stephen W WilsonThe mechanisms that regulate sarcomere assembly during myofibril formation are poorly understood. In this study, we characterise the zebrafish sloth(u45) mutant, in which the initial steps in sarcomere assembly take place, but thick filaments are absent and filamentous I-Z-I brushes fail to align or adopt correct spacing. The mutation only affects skeletal muscle and mutant embryos show no other obvious phenotypes. Surprisingly, we find that the phenotype is due to mutation in one copy of a tandemly duplicated hsp90a gene. The mutation disrupts the chaperoning function of Hsp90a through interference with ATPase activity. Despite being located only 2 kb from hsp90a, hsp90a2 has no obvious role in sarcomere assembly. Loss of Hsp90a function leads to the downregulation of genes encoding sarcomeric proteins and upregulation of hsp90a and several other genes encoding proteins that may act with Hsp90a during sarcomere assembly. Our studies reveal a surprisingly specific developmental role for a single Hsp90 gene in a regulatory pathway controlling late steps in sarcomere assembly.
History
Publication status
- Published
Journal
DevelopmentISSN
0950-1991Publisher
Company of BiologistsExternal DOI
Issue
6Volume
135Page range
1147-1156Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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