The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks

Loizou, Joanna I, El-Khamisy, Sherif F, Zlatanou, Anastasia, Moore, David J, Chan, Douglas W, Qin, Jun, Sarno, Stefania, Meggio, Flavio, Pinna, Lorenzo A and Caldecott, Keith W (2004) The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks. Cell, 117 (1). pp. 17-28. ISSN 0092-8674

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CK2 was the first protein kinase identified and is required for the proliferation and survival of mammalian cells. Here, we have identified an unanticipated role for CK2. We show that this essential protein kinase phosphorylates the scaffold protein XRCC1 and thereby enables the assembly and activity of DNA single-strand break repair protein complexes in vitro and at sites of chromosomal breakage. Moreover, we show that inhibiting XRCC1 phosphorylation by mutation of the CK2 phosphorylation sites or preventing CK2 activity using a highly specific inhibitor ablates the rapid repair of cellular DNA single-strand breaks by XRCC1. These data identify a direct role for CK2 in the repair of chromosomal DNA strand breaks and in maintaining genetic integrity.

Item Type: Article
Additional Information: Work was conducted in my laboraotory, by my student (JL)- External authors provided some materials. Identified a novel role for casein kinase in DNA repair.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Sherif El-Khamisy
Date Deposited: 06 Feb 2012 20:34
Last Modified: 02 Apr 2012 11:17
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