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Asymmetric Arginine dimethylation of Epstein–Barr virus nuclear antigen 2 promotes DNA targeting
journal contribution
posted on 2023-06-08, 07:08 authored by Henrik Gross, Stephanie Barth, Richard D Palermo, Alfredo Mamiani, Christine Hennard, Ursula Zimber-Strobl, Michelle WestMichelle West, Elisabeth Kremmer, Friedrich A GrasserThe Epstein-Barr virus (EBV) growth-transforms B-lymphocytes. The virus-encoded nuclear antigen 2 (EBNA2) is essential for transformation and activates gene expression by association with DNA-bound transcription factors such as RBPJk (CSL/CBF1). We have previously shown that EBNA2 contains symmetrically dimethylated Arginine (sDMA) residues. Deletion of the RG-repeat results in a reduced ability of the virus to immortalise B-cells. We now show that the RG repeat also contains asymmetrically dimethylated Arginines (aDMA) but neither non-methylated (NMA) Arginines nor citrulline residues. We demonstrate that only aDMA-containing EBNA2 is found in a complex with DNA-bound RBPJk in vitro and preferentially associates with the EBNA2-responsive EBV C, LMP1 and LMP2A promoters in vivo. Inhibition of methylation in EBV-infected cells results in reduced expression of the EBNA2-regulated viral gene LMP1, providing additional evidence that methylation is a prerequisite for DNA-binding by EBNA2 via association with the transcription factor RBPJk.
History
Publication status
- Published
Journal
VirologyISSN
0042-6822Publisher
ElsevierExternal DOI
Issue
2Volume
397Page range
299-310Pages
12.0Department affiliated with
- Biochemistry Publications
Research groups affiliated with
- Haematology Research Group Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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