Saccharomyces cerevisiae histone H2A Ser122 facilitates DNA repair.

Harvey, Anne C, Jackson, Stephen P and Downs, Jessica A (2005) Saccharomyces cerevisiae histone H2A Ser122 facilitates DNA repair. Genetics, 170. pp. 543-553. ISSN 0016-6731

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DNA repair takes place in the context of chromatin. Recently, it has become apparent that proteins that make up and modulate chromatin structure are involved in the detection and repair of DNA lesions. We previously demonstrated that Ser129 in the carboxyl-terminal tail of yeast histone H2A is important for double-strand-break responses. By undertaking a systematic site-directed mutagenesis approach, we identified another histone H2A serine residue (Ser122) that is important for survival in the presence of DNA-damaging agents. We show that mutation of this residue does not affect DNA damage-dependent Rad53 phosphorylation or G2/M checkpoint responses. Interestingly, we find that yeast lacking H2A S122 are defective in their ability to sporulate. Finally, we demonstrate that H2A S122 provides a function distinct from that of H2A S129. These data demonstrate a role for H2A S122 in facilitating survival in the presence of DNA damage and suggest a potential role in mediating homologous recombination. The distinct roles of H2A S122 and S129 in mediating these responses suggest that chromatin components can provide specialized functions for distinct DNA repair and survival mechanisms and point toward the possibility of a complex DNA damage responsive histone code.

Item Type: Article
Additional Information: I designed and orchestrated this project, which was experimentally carried out entirely by my first PhD student. The work was initiated in the Jackson lab.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Jessica Downs
Date Deposited: 06 Feb 2012 20:39
Last Modified: 02 Apr 2012 13:56
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