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The phagocytic capacity of neurones
journal contribution
posted on 2023-06-08, 07:49 authored by Samantha Bowen, Davidson D Ateh, Katrin Deinhardt, Margeret M Bird, Karen M Price, Cathy S Baker, Joanna C Robson, Michael Swash, Wassim Shamsuddin, Shalini Kawar, Tariq El-Tawil, Jesper Roos, Andrew Hoyle, Carole D Nickols, Charles H Knowles, Anthony H Pullen, Phillip J Luthert, Roy O Weller, Majid HafezparastMajid Hafezparast, Robin J M Franklin, Tamas Revesz, Rosalind H M King, Otto Berninghausen, Elizabeth M C Fisher, Giampietro Schiavo, Joanne E MartinPhagocytosis is defined as the ingestion of particulates over 0.5 microm in diameter and is associated with cells of the immune system such as macrophages or monocytes. Neurones are not generally recognized to be phagocytic. Using light, confocal, time-lapse and electron microscopy, we carried out a wide range of in-vitro and in-vivo experiments to examine the phagocytic capacity of different neuronal cell types. We demonstrated phagocytosis of material by neurones, including cell debris and synthetic particles up to 2.8 microm in diameter. We showed phagocytosis in different neuronal types, and demonstrated that debris can be transported from neurite extremities to cell bodies and persist within neurones. Flow cytometry analysis demonstrated the lack of certain complement receptors on neurones but the presence of others, including integrin receptors known to mediate macrophage phagocytosis, indicating that a restricted set of phagocytosis receptors may mediate this process. Neuronal phagocytosis occurs in vitro and in vivo, and we propose that this is a more widespread and significant process than previously recognized. Neuronal phagocytosis may explain certain inclusions in neurones during disease, cell-to-cell spread of disease, neuronal death during disease progression and provide a potential mechanism for therapeutic intervention through the delivery of particulate drug carriers.
History
Publication status
- Published
Journal
European Journal of NeuroscienceISSN
0953-816XPublisher
Blackwell PublishingExternal DOI
Issue
10Volume
25Page range
2947-55Department affiliated with
- Neuroscience Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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