University of Sussex
Browse

File(s) not publicly available

Lipopolysaccharides from atherosclerosis-associated bacteria antagonize TLR4, induce formation of TLR2/1/CD36 complexes in lipid rafts and trigger TLR2-induced inflammatory responses in human vascular endothelial cells

journal contribution
posted on 2023-06-08, 08:05 authored by Martha Triantafilou, Frederick G J Gamper, Philipp M Lepper, Marios-Angelos Mouratis, Christian Schumann, Evlambia Harokopakis, Robert E Schifferle, George Hajishengallis, Kathy Triantafilou
Infection with bacteria such as Chlamydia pneumonia, Helicobacter pylori, or Porphyromonas gingivalis may be triggering the secretion of inflammatory cytokines that leads to atherogenesis. The mechanisms by which the innate immune recognition of these pathogens could lead to atherosclerosis remain unclear. In this study, using human vascular endothelial cells or HEK293 cells engineered to express pattern-recognition receptors (PRRs), we set out to determine Toll-like receptors (TLRs) and functionally associated PRRs involved in the innate recognition of and response to LPS from H. pylori or P. gingivalis. Using siRNA interference or recombinant expression of cooperating PRRs, we show that H. pylori and P. gingivalis LPS-induced cell activation is mediated through TLR2. Human vascular endothelial cell activation was found to be lipid-raft dependent and to require the formation of heterotypic receptor complexes comprising of TLR2, TLR1, CD36 and CD11b/CD18. In addition, we report that LPS from these bacterial strains are able to antagonise TLR4. This antagonistic activity of H. pylori or P. gingivalis LPS, as well as their TLR2 activation capability may be associated with their ability to contribute to atherosclerosis.

History

Publication status

  • Published

Journal

Cellular Microbiology

ISSN

1462-5822

Issue

8

Volume

9

Page range

2030-2039

Pages

10.0

Department affiliated with

  • Biochemistry Publications

Notes

Designed work, performed 80% of work, wrote paper.

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC