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The Epstein Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1

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posted on 2023-06-08, 08:33 authored by James Heather, Kirsty Flower, Samine Isaac, Alison Sinclair
Activation of the host gene egr1 is essential for the lytic replication of EpsteinBarr virus (EBV). egr1 is activated by Zta (BZLF1, ZEBRA). Zta interacts directly with DNA through a series of closely related Zta-response elements (ZREs). Here we dissect the mechanism used by Zta to interact with the egr1 promoter and identify a weak interaction with egr1ZRE that is dependent on the distal part of egr1ZRE. Furthermore, we demonstrate that the ability of Zta to interact with egr1ZRE is enhanced at least tenfold by methylation. The ability of Zta to transactivate a reporter construct driven by the egr1 promoter can be enhanced by methylation. As the ability of Zta to interact with a methylated ZRE in the EBV genome correlates with its ability to activate the expression of the endogenous viral gene BRLF1, this suggests that Zta may also have the capability to overturn epigenetic control of egr1.

History

Publication status

  • Published

File Version

  • Published version

Journal

Journal of General Virology

ISSN

0022-1317

Publisher

Society for General Microbiology

Issue

6

Volume

90

Page range

1450-1454

Pages

5.0

Department affiliated with

  • Biochemistry Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

First Open Access (FOA) Date

2017-11-15

First Compliant Deposit (FCD) Date

2017-11-15

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