University of Sussex
Browse

File(s) not publicly available

DNA polymerase ? is an A-T mutator in somatic hypermutation of immunoglobulin variable genes

journal contribution
posted on 2023-06-08, 08:44 authored by Xianmin Zeng, David B Winter, Cynthia Kasmer, Kenneth H Kraemer, Alan LehmannAlan Lehmann, Patricia J Gearhart
To determine whether DNA polymerase plays a role in the hypermutation of immunoglobulin variable genes, we examined the frequency and pattern of substitutions in variable VH6 genes from the peripheral blood lymphocytes of three patients with xeroderma pigmentosum variant disease, whose polymerase had genetic defects. The frequency of mutation was normal but the types of base changes were different: there was a decrease in mutations at A and T and a concomitant rise in mutations at G and C. We propose that more than one polymerase contributes to hypermutation and that if one is absent, others compensate. The data indicate that polymerase is involved in generating errors that occur predominantly at A and T and that another polymerase(s) may preferentially generate errors opposite G and C.

History

Publication status

  • Published

Journal

Nature Immunology

ISSN

1529-2908

Publisher

Nature Publishing Group

Volume

2

Page range

537-541

Pages

5.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC