Novel functional requirements for non-homologous DNA end joining in Schizosaccharomyces pombe

Manolis, Kostas G, Nimmo, Elaine R, Hartsuiker, Edgar, Carr, Antony M, Jeggo, Penny A and Allshire, Robin C (2001) Novel functional requirements for non-homologous DNA end joining in Schizosaccharomyces pombe. EMBO Journal, 20. pp. 210-221. ISSN 0261-4189

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DNA double strand break (DSB) repair by non-homologous end joining (NHEJ) in mammalian cells requires the Ku70¿Ku80 heterodimer, the DNA-PK catalytic subunit DNA-PKcs, as well as DNA ligase IV and Xrcc4. NHEJ of plasmid DSBs in Saccharomyces cerevisiae requires Ku, Xrcc4 and DNA ligase IV, as well as Mre11, Rad50, Xrs2 and DNA damage checkpoint proteins. Saccharomyces cerevisiae Ku is also required for telomere length maintenance and transcriptional silencing. We have characterized NHEJ in Schizosaccharomyces pombe using an extrachromosomal assay and find that, as anticipated, it is Ku70 and DNA ligase IV dependent. Unexpectedly, we find that Rad32, Rad50 (the S.pombe homologues of Mre11 and Rad50, respectively) and checkpoint proteins are not required for NHEJ. Furthermore, although S.pombe Ku70 is required for maintenance of telomere length, it is dispensable for transcriptional silencing at telomeres and is located throughout the nucleus rather than concentrated at the telomeres. Together, these results provide insight into the mechanism of NHEJ and contrast significantly with recent studies in S.cerevisiae.

Item Type: Article
Additional Information: I participated in the analysis of Rad50 in non homologous end joining by providing deletion strains and through extensive advice and discussions with the first author of the paper. We show that Rad50 is not involved in non-homologous end joining in S. pombe.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Edgar Hartsuiker
Date Deposited: 06 Feb 2012 21:07
Last Modified: 26 Jun 2012 14:49
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