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Nitric oxide, sepsis and liver metabolism

journal contribution
posted on 2023-06-08, 09:29 authored by E D Ceppi, F S Smith, Michael Titheradge
Treatment of rats with bacterial endotoxin (LPS) significantly lowers hepatic gluconeogenesis and induces nitric oxide (NO) synthase over 18 hours. The induction of NO synthase correlates with alterations in plasma nitrate plus nitrite, and also with an inhibition of glucose synthesis in subsequently isolated hepatocytes. Artifical NO donors similarly decrease glucose synthesis. Cultured hepatocytes treated with a mixture of LPS, interferon-¿, tumour necrosis factor-a and IL-1ß show a similar inhibition of glucose output and induction of NO synthase, although NO synthase inhibitors can only partially reverse the inhibition of glucose output. Inclusion of glucagon during the induction period diminishes the ability of the cytokines to induce NO synthase and attenuates the inhibitory effect on glucose output. The inhibitory effect of glucagon on the expression of NO synthase can be mimicked by dibutyryl cyclic AMP and forskolin.

History

Publication status

  • Published

Journal

Biochemical Society Transactions

ISSN

03005127

Issue

3

Volume

25

Page range

929-934

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

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