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Measuring cellular migration with image processing

journal contribution
posted on 2023-06-08, 11:21 authored by Constantino C Reyes-Aldasoro, D Biram, G M Tozer, C Kanthou
An image-processing algorithm for analysis of migration of vascular endothelial cells in culture is presented. The algorithm correctly detected the cellular regions on either side of an artificial 'wound' made by dragging a sterile pipette tip across the monolayer of cells (scratch wound assay). Frequency filtering and mathematical morphology were used to approximate the boundaries of cellular regions. This allowed measurement of the distance between the regions, and therefore the migration rates, regardless of the orientation of the wound and even in cases where the cells were sparse and not tightly packed.

History

Publication status

  • Published

Journal

Electronics Letters

ISSN

0013-5194

Publisher

Institute of Electrical and Electronics Engineers

Issue

13

Volume

44

Page range

791-793

Department affiliated with

  • Engineering and Design Publications

Notes

328GE Times Cited:4 Cited References Count:13

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-04-24

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