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Salt tolerance in the halophyte Suaeda maritima. Further properties of the enzyme malate dehydrogenase

journal contribution
posted on 2023-06-08, 11:31 authored by Tim Flowers, J L Hall, Margaret E Ward
Malate dehydrogenase activity in supernatant fractions prepared from the halophyte Suaeda maritima was modified by added NACl with an optimal concentration for activation of about 50 mM. At this ionic strength of 0.05 the chlorides of sodium, potassium, ammonium, rubidium, calcium and magnesium all produced a similar degree of stimulation, while the nitrates of potassium and sodium were somewhat less effective. A similar result was obtained whether the plants were grown in the presence or absence of NACl. Furthermore, malate dehydrogenase activity in preparations from the glycophyte Pisum sativum behaved in a similar manner. The enzyme activity from both Suaeda and Pisum was separable into two fractions (I and II) by gel filtration on Sephadex G200. The MW of fraction II from Suaeda was estimated to be 165000 and that from Pisum approximately 282000: fraction I from both species eluted at the void volume of Sephadex G200. Storage of lyophilised supernatant resulted in the loss of enzyme activity from fraction I and a decrease in the overall stimulation by NaCl. Treatment of the lyophilised enzyme with NACl at a concentration of 100 mM also resulted in the loss of enzyme activity from fraction I.

History

Publication status

  • Published

Journal

Phytochemistry

ISSN

0031-9422

Publisher

Elsevier

Issue

8

Volume

15

Page range

1231-1234

Department affiliated with

  • Evolution, Behaviour and Environment Publications

Notes

Times Cited: 16 Flowers, tj hall, jl ward, me

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-05-16

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