Improvement of quantitation of biological X-ray microanalysis

Harvey, Diane M R, Flowers, T J and Kent, B (1984) Improvement of quantitation of biological X-ray microanalysis. Journal Of Microscopy, 134 (APR). pp. 93-100. ISSN 0022-2720

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Abstract

Absolute measurements of elemental concentrations within thin biological samples are often made by reference to a series of standards which resemble the samples in chemical and physical properties and the linear relationship between (p-b)/c and concentration. This principle requires that the chemical and physical properties of the matrix remain constant throughout a series of standards with different elemental contents and throughout different regions of the samples. Some of the changes undergone by specimens during X-ray microanalysis, e.g. loss of elements or organic mass loss, are also influenced by the composition of the matrix. A simple empirical modification to the linear (p-b)/c versus concentration relationship is presented to account for some of these effects and therefore improve quantitation of analyses.

Item Type: Article
Additional Information: Times Cited: 16 Harvey, dmr flowers, tj kent, b
Keywords: X-ray microanalysis, biological samples, quantitation, standards, properties of matrix, loss of element of interest, mass loss, contamination, minimum detectable concentration, non-linear calibration
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Subjects: Q Science
Depositing User: Catrina Hey
Date Deposited: 16 May 2012 08:46
Last Modified: 30 Nov 2012 17:13
URI: http://srodev.sussex.ac.uk/id/eprint/39167
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