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Translation initiation factors and active sites of protein synthesis co-localize at the leading edge of migrating fibroblasts

journal contribution
posted on 2023-06-08, 12:30 authored by Mark Willett, Michele Brocard, Alexandre Davide, Simon Morley
Cell migration is a highly controlled essential cellular process, often dysregulated in tumour cells, dynamically controlled by the architecture of the cell. Studies involving cellular fractionation and microarray profiling have previously identified functionally distinct mRNA populations specific to cellular organelles and architectural compartments. However, the interaction between the translational machinery itself and cellular structures is relatively unexplored. To help understand the role for the compartmentalization and localized protein synthesis in cell migration, we have used scanning confocal microscopy, immunofluorescence and a novel ribopuromycylation method to visualize translating ribosomes. In the present study we show that eIFs (eukaryotic initiation factors) localize to the leading edge of migrating MRC5 fibroblasts in a process dependent on TGN (trans-Golgi network) to plasma membrane vesicle transport. We show that eIF4E and eIF4GI are associated with the Golgi apparatus and membrane microdomains, and that a proportion of these proteins co-localize to sites of active translation at the leading edge of migrating cells.

History

Publication status

  • Published

File Version

  • Published version

Journal

The Biochemical Journal

ISSN

1470-8728

Publisher

Portland Press

Issue

1

Volume

438

Page range

217-227

Department affiliated with

  • Biochemistry Publications

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-10-29

First Compliant Deposit (FCD) Date

2017-03-07

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