Cdk activity couples epigenetic centromere inheritance to cell cycle progression

Silva, Marina C C, Bodor, Dani L, Madison, Stellfox E, Martins, Nuno M C, Hochegger, Helfrid, Foltz, Daniel R and Jansen, Lars E T (2012) Cdk activity couples epigenetic centromere inheritance to cell cycle progression. Developmental Cell, 22 (1). pp. 52-63. ISSN 1534-5807

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Centromeres form the site of chromosome attachment to microtubules during mitosis. Identity of these loci is maintained epigenetically by nucleosomes containing the histone H3 variant CENP-A. Propagation of CENP-A chromatin is uncoupled from DNA replication initiating only during mitotic exit. We now demonstrate that inhibition of Cdk1 and Cdk2 activities is sufficient to trigger CENP-A assembly throughout the cell cycle in a manner dependent on the canonical CENP-A assembly machinery. We further show that the key CENP-A assembly factor Mis18BP1(HsKNL2) is phosphorylated in a cell cycle-dependent manner that controls its centromere localization during mitotic exit. These results strongly support a model in which the CENP-A assembly machinery is poised for activation throughout the cell cycle but kept in an inactive noncentromeric state by Cdk activity during S, G2, and M phases. Alleviation of this inhibition in G1 phase ensures tight coupling between DNA replication, cell division, and subsequent centromere maturation

Item Type: Article
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Subjects: Q Science
Depositing User: Deeptima Massey
Date Deposited: 01 Nov 2012 09:32
Last Modified: 01 Nov 2012 09:32
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