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Apolipoprotein E (apoE) isoforms differentially induce nitric oxide production in endothelial cells
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posted on 2023-06-08, 12:39 authored by Sandra SacreSandra Sacre, Anita K Stannard, James S OwenAlthough apolipoprotein E3 (apoE3) is atheroprotective, two common isoforms, apoE2 and apoE4, produce recessive and dominant hyperlipidaemias, respectively. Using a fluorescent assay, we report herein that apoE3 particles secreted from recombinant cells stimulate more nitric oxide release in cultured human EA.hy926 endothelial cells than apoE2 or apoE4 (141% more than controls vs. 61 or 11%). Phosphatidylinositol (PI) 3-kinase inhibitors suppressed the apoE effect, while apoE receptor 2 (apoER2) was tyrosine phosphorylated. We conclude that apoE stimulates endothelial nitric oxide release in an isoform-dependent manner, and propose that tyrosine phosphorylation of apoER2 initiates PI3-kinase signalling and activation of nitric oxide synthase.
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Publication status
- Published
Journal
FEBS LettersISSN
0014-5793Publisher
ElsevierExternal DOI
Issue
1-3Volume
540Page range
181-187Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-11-01Usage metrics
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