Sacre, Sandra M, Stannard, Anita K and Owen, James S (2003) Apolipoprotein E (apoE) isoforms differentially induce nitric oxide production in endothelial cells. FEBS Letters, 540 (1-3). pp. 181-187. ISSN 0014-5793
Full text not available from this repository.Abstract
Although apolipoprotein E3 (apoE3) is atheroprotective, two common isoforms, apoE2 and apoE4, produce recessive and dominant hyperlipidaemias, respectively. Using a fluorescent assay, we report herein that apoE3 particles secreted from recombinant cells stimulate more nitric oxide release in cultured human EA.hy926 endothelial cells than apoE2 or apoE4 (141% more than controls vs. 61 or 11%). Phosphatidylinositol (PI) 3-kinase inhibitors suppressed the apoE effect, while apoE receptor 2 (apoER2) was tyrosine phosphorylated. We conclude that apoE stimulates endothelial nitric oxide release in an isoform-dependent manner, and propose that tyrosine phosphorylation of apoER2 initiates PI3-kinase signalling and activation of nitric oxide synthase.
Item Type: | Article |
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Schools and Departments: | Brighton and Sussex Medical School > Clinical and Experimental Medicine |
Subjects: | Q Science R Medicine |
Depositing User: | Sandra Sacre |
Date Deposited: | 01 Nov 2012 12:02 |
Last Modified: | 05 Oct 2017 18:27 |
URI: | http://srodev.sussex.ac.uk/id/eprint/41164 |