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Cyclosporin A and tacrolimus reduce T-cell polyfunctionality but not interferon-gamma responses directed at cytomegalovirus
journal contribution
posted on 2023-06-08, 12:41 authored by S Fuhrmann, R Lachmann, M Streitz, R Hetzer, H D Volk, H Lehmkuhl, Florian KernFlorian KernCytomegalovirus (CMV) -specific immunity is often estimated by the number of in vitro CMV antigen-inducible interferon-gamma-positive (IFN-gamma(+) ) T cells. However, recent work indicates that simultaneous production of IFN-gamma, tumour necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) (referred to as 'polyfunctionality') is more relevant for anti-viral protection. Here, we compared polyfunctionality of CMV-specific T cells (pp65 and IE-1 proteins) in 23 solid-organ transplant patients and seven healthy controls by flow cytometry. The proportions of TNF-alpha(+) /IFN-gamma(+) /IL-2 cells among the activated cells were significantly reduced in transplant patients but not the frequencies of IFN-gamma(+) CD8(+) T cells. Immunosuppression reduces polyfunctionality, which reflects the increased infection risk in this patient group.
History
Publication status
- Published
Journal
ImmunologyISSN
0019-2805Publisher
Blackwell PublishingExternal DOI
Issue
4Volume
136Page range
408-413Department affiliated with
- Clinical and Experimental Medicine Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-10-22Usage metrics
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No categories selectedKeywords
Adolescent; Adult; CD8-Positive T-Lymphocytes/drug effects/*immunology/metabolismCyclosporine/*pharmacologyCytomegalovirus/*immunologyCytomegalovirus Infections/*immunologyFemaleHumansImmunosuppressive Agents/*pharmacologyInterferon-gamma/*biosynthesisInterleukin-2/biosynthesisLymphocyte ActivationMaleMiddle AgedOrgan TransplantationTacrolimus/*pharmacologyTumor Necrosis Factor-alpha/biosynthesisYoung Adult
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