Caser Tena, Teresa, Maerz, Lars, Szafranski, Karol, Groth, Marco, Blatte, Tamara, Donow, Cornelia, Matysik, Sabrina, Walther, Paul, Jeggo, Penelope A, Burkhalter, Martin and Philipp, Melanie (2018) Resting cells rely on the DNA helicase component MCM2 to build cilia. Nucleic Acids Research. ISSN 0305-1048
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Abstract
Minichromosome maintenance (MCM) proteins facilitate replication by licensing origins and unwinding the DNA double strand. Interestingly, the number of MCM hexamers greatly exceeds the number of firing origins suggesting additional roles of MCMs. Here we show a hitherto unanticipated function of MCM2 in cilia formation in human cells and zebrafish that is uncoupled from replication. Zebrafish depleted of MCM2 develop ciliopathy-phenotypes including microcephaly and aberrant heart looping due to malformed cilia. In non-cycling human fibroblasts, loss of MCM2 promotes transcription of a subset of genes, which cause cilia shortening and centriole overduplication. Chromatin immunoprecipitation experiments show that MCM2 binds to transcription start sites of cilia inhibiting genes. We propose that such binding may block RNA polymerase II-mediated transcription. Depletion of a second MCM (MCM7), which functions in complex with MCM2 during its canonical functions, reveals an overlapping cilia-deficiency phenotype likely unconnected to replication, although MCM7 appears to regulate a distinct subset of genes and pathways. Our data suggests that MCM2 and 7 exert a role in ciliogenesis in post-mitotic tissues.
Item Type: | Article |
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Schools and Departments: | School of Life Sciences > Sussex Centre for Genome Damage and Stability |
Research Centres and Groups: | Genome Damage and Stability Centre |
Subjects: | Q Science > QH Natural history > QH0301 Biology > QH0426 Genetics Q Science > QH Natural history > QH0301 Biology |
Depositing User: | Penny Jeggo |
Date Deposited: | 19 Oct 2018 13:30 |
Last Modified: | 19 Oct 2018 14:19 |
URI: | http://srodev.sussex.ac.uk/id/eprint/41738 |
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📧 Request an updateProject Name | Sussex Project Number | Funder | Funder Ref |
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DNA damage responses in mammalian cells and their contribution to human health disorders; the end-stage | G0217 | MRC-MEDICAL RESEARCH COUNCIL | G1000050 |