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Identification of circulating microRNAs as diagnostic biomarkers for use in multiple myeloma

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posted on 2023-06-08, 13:27 authored by Chris JonesChris Jones, M V Zabolotskaya, A J King, Helen Stewart, G A Horne, Timothy ChevassutTimothy Chevassut, Sarah NewburySarah Newbury
BACKGROUND: Multiple myeloma is a plasma cell disorder that is characterised by clonal proliferation of malignant plasma cells in the bone marrow, monoclonal paraprotein in the blood or urine and associated organ dysfunction. It accounts for approximately 1% of cancers and 13% of haematological cancers. Myeloma arises from an asymptomatic proliferation of monoclonal plasma cells termed monoclonal gammopathy of undetermined significance (MGUS). METHODS: MicroRNA expression profiling of serum samples was performed on three patient groups as well as normal controls. Validation of the nine microRNAs detected as promising biomarkers was carried out using TaqMan quantitative RT-PCR. MicroRNA levels in serum were normalised using standard curves to determine the numbers of microRNAs per µl of serum. RESULTS: Three serum microRNAs, miR-720, miR-1308 and miRNA-1246, were found to have potential as diagnostic biomarkers in myeloma. Use of miR-720 and miR-1308 together provides a powerful diagnostic tool for distinguishing normal healthy controls, as well as patients with unrelated illnesses, from precancerous myeloma and myeloma patients. In addition, the combination of miR-1246 and miR-1308 can distinguish MGUS from myeloma patients. CONCLUSION: We have developed a biomarker signature using microRNAs extracted from serum which has potential as a diagnostic and prognostic tool for multiple myeloma.

Funding

Targeted mRNA degradation in Drosophila spermatogenosis; G0045; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/I007989/1

Epigenetic regulation of gene expression by the exoribonuclease pacman; G0056; BBSRC-BIOTECHNOLOGY & BIOLOGICAL SCIENCES RESEARCH COUNCIL; BB/I021345/1

History

Publication status

  • Published

File Version

  • Published version

Journal

British Journal of Cancer

ISSN

0007-0920

Publisher

Nature Publishing Group

Issue

12

Volume

107

Page range

1987-1996

Department affiliated with

  • Clinical and Experimental Medicine Publications

Research groups affiliated with

  • Haematology Research Group Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2013-03-14

First Open Access (FOA) Date

2016-03-08

First Compliant Deposit (FCD) Date

2016-03-08

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