Tumor necrosis factor-alpha drives HIV-1 replication in U937 cell clones and upregulates CXCR4

Biswas, Priscilla, Mantelli, Barbara, Delfanti, Fanny, Cota, Manuela, Vallanti, Giuliana, de Filippi, Camilla, Mengozzi, Manuela, Vicenzi, E, Lazzarin, A and Poli, Guido (2001) Tumor necrosis factor-alpha drives HIV-1 replication in U937 cell clones and upregulates CXCR4. Cytokine, 13 (1). pp. 55-59. ISSN 1043-4666

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U937 cell clones in which efficient (plus) vs poor (minus) replication of HIV-1 occurs have been described. We evaluated the role of host factors in their differential ability to support HIV-1 replication. Plus clones constitutively produced TNF-alpha and viral replication was inhibited by neutralization of endogenous TNF-alpha. However, HIV-1 replication was strongly upregulated in minus clones by exogenous TNF-alpha, which also further accelerated the kinetics of infection in plus clones. We observed an increased accumulation of proviral DNA within one round of HIV-1 replication following TNF-a treatment of plus cells. This effect was associated with increased surface density of CXCR4 in both plus and minus clones. Our results identify TNF-alpha as one correlate that contributes to the higher ability of U937-plus clones to sustain HIV-1 replication. Furthermore, we suggest that TNF-alpha may affect steps of the viral life cycle that occur earlier than transcription and also enhance HIV-1 replication by increasing the surface density of CXCR4

Item Type: Article
Keywords: cytokines, inflammation, chemokines
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Subjects: Q Science > Q Science (General)
Depositing User: Manuela Mengozzi
Date Deposited: 14 Nov 2012 08:40
Last Modified: 05 Oct 2017 18:27
URI: http://srodev.sussex.ac.uk/id/eprint/42340
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