Inactivation of the complement anaphylatoxin C5a by secreted products of parasitic nematodes

Rees-Roberts, Dominic, Mullen, Lisa M, Gounaris, Kleoniki and Selkirk, Murray E (2010) Inactivation of the complement anaphylatoxin C5a by secreted products of parasitic nematodes. International Journal for Parasitology, 40 (5). pp. 527-532. ISSN 0020-7519

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Given the importance of the complement anaphylatoxins in cellular recruitment during infection, the ability of secreted products from larval stages of Brugia malayi and Trichinella spiralis to influence C5a-mediated chemotaxis of human peripheral blood granulocytes in vitro was examined. Secreted products from B. malayi microfilariae almost completely abolished chemotaxis. This inhibition was blocked by phenylmethylsulphonyl fluoride, indicating the presence of a serine protease, which was subsequently shown to cleave C5a. In contrast, secreted products from T. spiralis infective larvae showed modest inhibition of C5a-mediated granulocyte chemotaxis, and this was blocked by potato carboxypeptidase inhibitor, an inhibitor of several metallocarboxypeptidases. Adult and larval stages of both parasites were demonstrated to secrete carboxypeptidases which cleaved hippuryl-L-lysine and hippuryl-L-arginine, and the T. spiralis enzyme was partially characterised. The data are discussed with reference to inflammation in parasitic nematode infection.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Subjects: Q Science > QR Microbiology > QR0180 Immunology
Depositing User: Lisa Mullen
Date Deposited: 18 Jan 2013 11:09
Last Modified: 05 Oct 2017 18:26
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