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The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections
journal contribution
posted on 2023-06-08, 14:43 authored by Barry Panaretou, Kirsty Sinclair, Chrisostomos ProdromouChrisostomos Prodromou, Jasvinder Johal, Laurence PearlLaurence Pearl, Peter W PiperDuring infections with a number of important eukaryotic pathogens the Hsp90 molecular chaperone of the pathogen is recognized as an immunodominant antigen by the host immune system. Yeast molecular genetics should allow study of the extent of sequence variation within conserved immunodominant epitopes on pathogen Hsp90s that is compatible with essential Hsp90 functions, as well as the processes that generate antigenic subfragments of these Hsp90s. The Hsp90 of the fungal pathogen Candida albicans was shown in this study to provide both essential and nonessential (pheromone signalling and mammalian steroid receptor activation) Hsp90 functions in Saccharomyces cerevisiae cells. Much of the C. albicans Hsp90 expressed in respiratory S. cerevisiae cells was shown to undergo a partial degradation in vivo, a degradation that closely resembles that of the native Hsp82 (one isoform of the homologous Hsp90) in S. cerevisiae. Allowing for the differences in the length of the charged linker region between the N- and C-terminal domains of C. albicans Hsp90 and S. cerevisiae Hsp82, these two proteins expressed in S. cerevisiae appear to give the same major degradation products. These Hsp90 fragments are similar to the products of incomplete Hsp90 degradation found in C. albicans cultures.
History
Publication status
- Published
Journal
MicrobiologyISSN
1350-0872Publisher
Society for General MicrobiologyIssue
12Volume
145Page range
3455-3463Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2015-02-25Usage metrics
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No categories selectedKeywords
AntigensFungal/immunologyBlottingWesternCandida albicans/*genetics/metabolismCandidiasis/immunology/metabolismHSP90 Heat-Shock Proteins/*genetics/immunology/*metabolismHeat-Shock Proteins/metabolismHeat-Shock ResponsePeptide Fragments/immunologyPheromones/metabolismReceptorsGlucocorticoid/metabolismSaccharomyces cerevisiae/*genetics/growth & development/*metabolismSaccharomyces cerevisiae ProteinsSignal Transduction
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